Sensitivity of PCR in detecting monoclonal B cell proliferations.
نویسندگان
چکیده
منابع مشابه
Sensitivity of PCR in detecting monoclonal B cell proliferations.
AIMS To evaluate the rapid detection of various forms of monoclonal B cell proliferations by using the polymerase chain reaction (PCR) to identify clonal immunoglobulin heavy chain genomic rearrangements. METHODS Thirty four B cell lymphomas defined by morphology, immunophenotyping, and positive immunoglobulin heavy chain gene rearrangements detected by Southern blot analysis were examined. A...
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AIMS To develop a simple, optimised, polymerase chain reaction (PCR) based method for detecting the rearranged immunoglobulin heavy chain (IgH). METHODS Using as primers oligonucleotides (Fr2A, Fr2B) homologous to the conserved sequences to the framework II region and the joining (JH) region, 25 patients with B cell lymphoproliferative disorders, previously characterised by Southern blotting,...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1993
ISSN: 0021-9746
DOI: 10.1136/jcp.46.7.624